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Leonard Nimoy, the actor who starred as Spock in most of the character's appearances, can be seen in animated form in "The Springfield Files", an episode of The Simpsons that also features animated versions of Mulder and Scully.
A scene in the film Star Trek II: The Wrath of Khan which features the death of Spock, is deliberately referenced in a scene from The X-Files episode "Jump the Shark", in which the Lone Gunmen die. In the movie, Spock talked to his commanding officer and friend, Captain Kirk, through a wall of glass while dying. In The X-Files episode, Jimmy Bond speaks to the Lone Gunmen through a pane of glass shortly before they die.